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CompBioBench item

histone-chip-q1

Question

I have a histone ChIP-seq ENCODE-style filtered tagAlign file (histone.chip.q1.signal.tagalign.gz) along with its control (histone.chip.q1.control.tagalign.gz), both hg38. I lost the metadata. Can you figure out the histone mark? Output exactly one label from this set: H3K4me3, H3K27ac, H3K4me1, H3K36me3, H3K27me3, H3K9me3.

Inferred answer
H3K9me3Uniquely inferred from submitted scores.
Domain
Epigenomics
Question style
Metadata Recovery

An answer can be uniquely inferred for 54 of 100 questions from submitted scores. Green: correct · Red: incorrect. For questions without a uniquely determined answer: Blue: matches prediction · Orange: differs from prediction. These are inferred answer checks, not official per-question grades.

Galaxy

12/12 terminal item results

UDT = user-defined tool: custom code executed by Galaxy. Route badges summarize analysis tools, excluding uploads and automatic conversions. How usage is counted.

Codex GPT-5.5

3 replicates
Codex GPT-5.5

Replicate 1

CorrectUDT-only
AnswerH3K9me3
Workflow
Histone mark diagnosticHistone mark diagnostic fullHistone mark repeat contextHistone mark final label
Total tokens
935.8K
Output
16.5K
Codex GPT-5.5

Replicate 2

CorrectUDT + standard tools
AnswerH3K9me3
Workflow
Histone mark diagnostic chr1 chr2UDT render probeUDT render probe commandWc GnuGrep2 more tools
Total tokens
2.3M
Output
23.1K
Codex GPT-5.5

Replicate 3

CorrectUDT + standard tools
AnswerH3K9me3
Workflow
ChIP Histone Mark DiagnosticChIP Histone Mark Diagnostic 2ChIP Histone Mark Diagnostic 3ChIP Histone Mark Diagnostic 4Macs2 Callpeak3 more tools
Total tokens
3.3M
Output
23.6K

Codex GPT-5.6 Sol

3 replicates
Codex GPT-5.6 Sol

Replicate 1

CorrectUDT-only
AnswerH3K9me3
Workflow
Histone chr1 multiscale diagnosticHistone chr1 RefSeq profileHistone mark final classification
Total tokens
1.5M
Output
13.5K
Codex GPT-5.6 Sol

Replicate 2

CorrectUDT-only
AnswerH3K9me3
Workflow
TagAlign content probe q1hg38 histone signature diagnostics q1Validated histone mark label q1
Total tokens
5.1M
Output
20.9K
Codex GPT-5.6 Sol

Replicate 3

CorrectUDT-only
AnswerH3K9me3
Workflow
Chr1 histone mark diagnosticGenome-wide broad repressive histone diagnosticValidate broad domains against UCSC hg38 centromeresValidate broad domains against UCSC hg38 acen cytobandsFinal evidence-gated histone mark label
Total tokens
3.2M
Output
19.6K

Codex + DeepSeek-v4-pro-0813

3 replicates
Codex + DeepSeek-v4-pro-0813

Replicate 1

IncorrectStandard tools only
AnswerH3K4me1
Workflow
Macs2 CallpeakChipseekerDatamash OpsAdd A Column1Bedtools Bedtobam6 more tools
Total tokens
19.4M
Output
73.3K
Codex + DeepSeek-v4-pro-0813

Replicate 2

RerunIncorrectStandard tools only
AnswerH3K27ac
Workflow
macs2 callpeak → chipseeker → Count1 → Add a column1 → Summary Statistics1
Total tokens
12.6M
Output
45.3K
Codex + DeepSeek-v4-pro-0813

Replicate 3

IncorrectStandard tools only
AnswerH3K4me1
Workflow
Macs2 CallpeakChipseekerDatamash OpsGroup and aggregateCount111 more tools
Total tokens
11.0M
Output
46.7K

Codex GPT-5.6 Luna

3 replicates
Codex GPT-5.6 Luna

Replicate 1

CorrectUDT + standard tools
AnswerH3K9me3
Workflow
TagAlign distribution diagnosticTagAlign keyed-bin diagnosticMacs2 CallpeakMACS2 peak summaryMACS2 broad peak summary18 more tools
Total tokens
22.6M
Output
103.5K
Codex GPT-5.6 Luna

Replicate 2

CorrectUDT + standard tools
AnswerH3K9me3
Workflow
Macs2 RandsampleMacs2 CallpeakTagAlign diagnosticTagAlign gene profileTagAlign repeat profile1 more tools
Codex GPT-5.6 Luna

Replicate 3

CorrectUDT + standard tools
AnswerH3K9me3
Workflow
Upload1Macs2 CallpeakHistone ChIP-seq mark diagnosticsChipseekerDatamash Ops11 more tools
Total tokens
18.9M
Output
73.9K

Anycode

13/13 terminal item results

Codex GPT-5.5

3 replicates
Codex GPT-5.5

Replicate 1

Correct
AnswerH3K9me3
Workflow
Local codeBedtoolsPython
Codex GPT-5.5

Replicate 2

Correct
AnswerH3K9me3
Workflow
Local codeBedtoolsSamtoolsPythonpysam
Codex GPT-5.5

Replicate 3

Correct
AnswerH3K9me3
Workflow
Local codeBedtoolsPython

Codex GPT-5.6 Sol

3 replicates
Codex GPT-5.6 Sol

Replicate 1

Correct
AnswerH3K9me3
Workflow
Local codeBedtoolsSamtoolsPythonpysam
Codex GPT-5.6 Sol

Replicate 2

Correct
AnswerH3K9me3
Workflow
Local codeBedtoolsSamtoolsPythonR2 more tools
Codex GPT-5.6 Sol

Replicate 3

Correct
AnswerH3K9me3
Workflow
Local codeBedtoolsSamtoolsPythonSciPy

Codex + DeepSeek-v4-pro-0813

3 replicates
Codex + DeepSeek-v4-pro-0813

Replicate 1

Correct
AnswerH3K9me3
Workflow
Local codeBedtoolsSamtoolsPythonSciPy1 more tools
Total tokens
3.5M
Output
28.0K
Codex + DeepSeek-v4-pro-0813

Replicate 2

Correct
AnswerH3K9me3
Workflow
Local codeSamtoolsBedtoolsPython
Total tokens
7.1M
Output
44.3K
Codex + DeepSeek-v4-pro-0813

Replicate 3

Correct
AnswerH3K9me3
Workflow
Local codeBedtoolsSamtoolsPythonpysam1 more tools
Total tokens
5.3M
Output
51.5K

Codex GPT-5.6 Luna

3 replicates
Codex GPT-5.6 Luna

Replicate 1

Correct
AnswerH3K9me3
Workflow
Local codeBedtoolsSamtoolsPythonSciPy1 more tools
Codex GPT-5.6 Luna

Replicate 2

Correct
AnswerH3K9me3
Workflow
Local codePythonBedtoolsSamtoolspysam1 more tools
Codex GPT-5.6 Luna

Replicate 3

Correct
AnswerH3K9me3
Workflow
Local codeBedtoolsSamtoolsPythonSciPy1 more tools

Codex GPT-6 Astra

1 replicate
Codex GPT-6 Astra

Replicate 1

Correct
AnswerH3K9me3
Workflow
Local codePythonSciPypysamBedtools
Total tokens
332.7K
Output
4.3K