Question
I have FASTQ data from a perturbation experiment involving KLF4 exogenous overexpression using a Sendai virus. I want to estimate the fraction of exogenous-origin reads in exogenous.mix.reads.q2.mix.fq, given exogenous.mix.reads.q2.exo.fq (early timepoint, assumed purely exogenous) and exogenous.mix.reads.q2.endo.fq (later timepoint, predominantly endogenous). All files contain reads mapping to KLF4. I don't have details on the Sendai construct. If possible, estimate the percent of exogenous reads in exogenous.mix.reads.q2.mix.fq (by read count) to the nearest 10 and return only the integer (e.g. 50); if not possible, return NA.
- Inferred answer
NAUniquely inferred from submitted scores.
- Domain
- Transcriptomics
- Question style
- Synthetic/Augmented Data
An answer can be uniquely inferred for 54 of 100 questions from submitted scores. Green: correct · Red: incorrect. For questions without a uniquely determined answer: Blue: matches prediction · Orange: differs from prediction. These are inferred answer checks, not official per-question grades.
Galaxy
12/12 terminal item results
UDT = user-defined tool: custom code executed by Galaxy. Route badges summarize analysis tools, excluding uploads and automatic conversions. How usage is counted.
Codex GPT-5.5
3 replicates
Codex GPT-5.5
Replicate 1
IncorrectUDT-only
Answer90
WorkflowKLF4 exogenous diagnostic→KLF4 exogenous diagnostic inline→KLF4 FASTQ overlap order diagnostic→KLF4 segment private kmer diagnostic→KLF4 final exogenous percent
- Total tokens
- 792.1K
- Output
- 16.4K
Codex GPT-5.5
Replicate 2
IncorrectUDT + standard tools
Answer90
WorkflowKLF4 exogenous mixture estimate→KLF4 exogenous mixture diagnostics v2→KLF4 exogenous mixture diagnostics v3→UDT render probe v1→UDT render probe v2→6 more tools
- Total tokens
- 3.6M
- Output
- 18.0K
Codex GPT-5.5
Replicate 3
IncorrectUDT + standard tools
Answer90
WorkflowKLF4 Origin Diagnostic→KLF4 Origin Diagnostic v2→KLF4 Origin Diagnostic v3→KLF4 Render Probe→KLF4 Render Probe 2→8 more tools
- Total tokens
- 4.4M
- Output
- 24.3K
Codex GPT-5.6 Sol
3 replicates
Codex GPT-5.6 Sol
Replicate 1
IncorrectUDT-only
Answer90
WorkflowKLF4 FASTQ source diagnostic→KLF4 exogenous read fraction estimator
- Total tokens
- 432.5K
- Output
- 7.9K
Codex GPT-5.6 Sol
Replicate 2
CorrectUDT-only
AnswerNA
WorkflowKLF4 read signature diagnostic→KLF4 read nesting diagnostic→KLF4 exogenous fraction final result
- Total tokens
- 1.1M
- Output
- 9.4K
Codex GPT-5.6 Sol
Replicate 3
CorrectUDT-only
AnswerNA
WorkflowKLF4 FASTQ sequence diagnostic→KLF4 FASTQ file relation
- Total tokens
- 536.2K
- Output
- 6.3K
Codex + DeepSeek-v4-pro-0813
3 replicates
Codex + DeepSeek-v4-pro-0813
Replicate 1
IncorrectStandard tools only
Answer90
WorkflowFastq To Tabular→Select columns→Wc Gnu→Comp1→Tp Sorted Uniq→2 more tools
- Total tokens
- 16.2M
- Output
- 31.6K
Codex + DeepSeek-v4-pro-0813
Replicate 2
CorrectUDT + standard tools
AnswerNA
WorkflowCshl Fastq To Fasta→Cshl Fastx Collapser→KLF4 mix k-mer diagnostic→Probe Minimal→Meryl Count Kmers→11 more tools
- Total tokens
- 27.7M
- Output
- 102.6K
Codex + DeepSeek-v4-pro-0813
Replicate 3
CorrectUDT + standard tools
AnswerNA
WorkflowCshl Fastx Collapser→Read overlap diagnostic→Fastq To Tabular→Group and aggregate→Summary Statistics1→1 more tools
- Total tokens
- 14.6M
- Output
- 76.5K
Codex GPT-5.6 Luna
3 replicates
Codex GPT-5.6 Luna
Replicate 1
CorrectUDT + standard tools
AnswerNA
WorkflowFastq To Tabular→KLF4 origin sequence diagnostics→KLF4 origin sequence profile→KLF4 origin sequence holdout test→KLF4 origin overlap diagnostic→3 more tools
- Total tokens
- 3.2M
- Output
- 29.5K
Codex GPT-5.6 Luna
Replicate 2
CorrectUDT + standard tools
AnswerNA
WorkflowCshl Fastx Collapser→Fastq Stats→Rnaspades→KLF4 origin diagnostic→KLF4 common-reference diagnostic→6 more tools
Codex GPT-5.6 Luna
Replicate 3
CorrectStandard tools only
AnswerNA
WorkflowFastq Stats→Fastq To Tabular→Comp1→Datasets Download Gene→Minimap2→6 more tools
- Total tokens
- 21.4M
- Output
- 62.3K