Question
I have FASTQ data from a perturbation experiment involving KLF4 exogenous overexpression using a Sendai virus. I want to estimate the fraction of exogenous-origin reads in exogenous.mix.reads.q1.mix.fq, given exogenous.mix.reads.q1.exo.fq (early timepoint, assumed purely exogenous) and exogenous.mix.reads.q1.endo.fq (later timepoint, predominantly endogenous). All files contain reads mapping to KLF4. I don't have details on the Sendai construct. If possible, estimate the percent of exogenous reads in exogenous.mix.reads.q1.mix.fq (by read count) to the nearest 10 and return only the integer (e.g. 50); if not possible, return NA.
- Inferred answer
30Uniquely inferred from submitted scores.
- Domain
- Transcriptomics
- Question style
- Synthetic/Augmented Data
An answer can be uniquely inferred for 54 of 100 questions from submitted scores. Green: correct · Red: incorrect. For questions without a uniquely determined answer: Blue: matches prediction · Orange: differs from prediction. These are inferred answer checks, not official per-question grades.
Galaxy
12/12 terminal item results
UDT = user-defined tool: custom code executed by Galaxy. Route badges summarize analysis tools, excluding uploads and automatic conversions. How usage is counted.
Codex GPT-5.5
3 replicates
Codex GPT-5.5
Replicate 1
IncorrectUDT-only
Answer50
WorkflowKLF4 Exogenous Mixture Diagnostics
- Total tokens
- 249.9K
- Output
- 5.8K
Codex GPT-5.5
Replicate 2
CorrectUDT + standard tools
Answer30
WorkflowFASTQ mix diagnostic→FASTQ mix diagnostic v2→FASTQ mix diagnostic v3→FASTQ mix diagnostic v4→UDT render probe→6 more tools
- Total tokens
- 2.4M
- Output
- 15.8K
Codex GPT-5.5
Replicate 3
IncorrectUDT + standard tools
Answer40
WorkflowKLF4 Exogenous Diagnostic→KLF4 Exogenous Diagnostic v2→UDT Render Probe Cat→UDT Render Probe Alt→Fastq To Tabular→4 more tools
- Total tokens
- 3.6M
- Output
- 20.1K
Codex GPT-5.6 Sol
3 replicates
Codex GPT-5.6 Sol
Replicate 1
CorrectUDT-only
Answer30
WorkflowKLF4 source diagnostic→KLF4 mixture model diagnostic→KLF4 final exogenous fraction
- Total tokens
- 1.1M
- Output
- 12.1K
Codex GPT-5.6 Sol
Replicate 2
CorrectUDT-only
Answer30
WorkflowKLF4 FASTQ empirical mixture diagnostic→KLF4 FASTQ exogenous fraction final estimator
- Total tokens
- 700.8K
- Output
- 11.1K
Codex GPT-5.6 Sol
Replicate 3
CorrectUDT + standard tools
Answer30
WorkflowKLF4 FASTQ signature diagnostic→KLF4 FASTQ signature diagnostic v2→KLF4 FASTQ signature diagnostic v3→Rnaspades→Bwa Mem→3 more tools
- Total tokens
- 6.1M
- Output
- 16.5K
Codex + DeepSeek-v4-pro-0813
3 replicates
Codex + DeepSeek-v4-pro-0813
Replicate 1
CorrectUDT + standard tools
Answer30
WorkflowCshl Fastx Collapser→Bwa Mem→Samtools View→Filter Tabular→Mix diagnostic→4 more tools
- Total tokens
- 5.4M
- Output
- 44.0K
Codex + DeepSeek-v4-pro-0813
Replicate 2
CorrectStandard tools only
Answer30
WorkflowFastq To Tabular→Group and aggregate→Count1
- Total tokens
- 12.8M
- Output
- 48.5K
Codex + DeepSeek-v4-pro-0813
Replicate 3
RerunCorrectUDT + standard tools
Answer30
WorkflowReference retrieval→Minimap2 alignment→Alignment summary→Mixture estimate
- Total tokens
- 3.1M
- Output
- 34.9K
Codex GPT-5.6 Luna
3 replicates
Codex GPT-5.6 Luna
Replicate 1
CorrectUDT + standard tools
Answer30
WorkflowSeqkit Split2→Fastq Stats→Megahit→Cat1→Tp Cat→14 more tools
- Total tokens
- 18.4M
- Output
- 61.3K
Codex GPT-5.6 Luna
Replicate 2
CorrectUDT + standard tools
Answer30
WorkflowKLF4 read diagnostic→KLF4 read diagnostic corrected→KLF4 mixture diagnostic→KLF4 mixture diagnostic corrected→KLF4 mixture diagnostic final diagnostic→8 more tools
- Total tokens
- 7.1M
- Output
- 51.4K
Codex GPT-5.6 Luna
Replicate 3
CorrectStandard tools only
Answer30
WorkflowFastq Stats→Cshl Fastx Collapser→Fasta Stats→Cshl Fastq To Fasta→Vsearch Search→6 more tools
- Total tokens
- 11.7M
- Output
- 46.7K