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CompBioBench item

atac-tn5-shift-q1

Question

I got a scATAC-seq fragment file (unknown.shift.frag.bed.gz) from a colleague who aligned it to the hg38 genome. However, they applied a non-standard shift to the reads instead of the typical +4/-5. Can you help figure out what it is? Report answer in the format "3;-3" (corresponding to values for 5' and 3' ends).

Inferred answer
2;-1Uniquely inferred from submitted scores.
Domain
Epigenomics
Question style
Metadata Recovery

An answer can be uniquely inferred for 54 of 100 questions from submitted scores. Green: correct · Red: incorrect. For questions without a uniquely determined answer: Blue: matches prediction · Orange: differs from prediction. These are inferred answer checks, not official per-question grades.

Galaxy

12/12 terminal item results

UDT = user-defined tool: custom code executed by Galaxy. Route badges summarize analysis tools, excluding uploads and automatic conversions. How usage is counted.

Codex GPT-5.5

3 replicates
Codex GPT-5.5

Replicate 1

IncorrectUDT-only
Answer2;-2
Workflow
Fragment summary diagnosticTn5 shift chr1 diagnosticTn5 shift multichrom final
Total tokens
525.8K
Output
11.0K
Codex GPT-5.5

Replicate 2

CorrectUDT-only
Answer2;-1
Workflow
ATAC shift diagnosticATAC shift diagnostic v2UDT smoke copy
Total tokens
1.8M
Output
19.1K
Codex GPT-5.5

Replicate 3

RerunIncorrectUDT-only
Answer6;-5
Workflow
ATAC shift endpoint sequence diagnosticATAC shift candidate scorerATAC shift HINT bias validationATAC shift HINT native kmer validation
Total tokens
1.7M
Output
24.6K

Codex GPT-5.6 Sol

3 replicates
Codex GPT-5.6 Sol

Replicate 1

CorrectUDT + standard tools
Answer2;-1
Workflow
Extract Genomic Dna 1Infer Tn5 shift from fragment-end sequence biasCreate centered fragment-end windowsCreate left fragment-end windowsLocate Tn5 bias axis in centered endpoint windows1 more tools
Codex GPT-5.6 Sol

Replicate 2

CorrectUDT + standard tools
Answer2;-1
Workflow
Make oriented endpoint windowsExtract Genomic Dna 1Analyze endpoint sequence biasCall nonstandard Tn5 shift
Total tokens
914.3K
Output
11.0K
Codex GPT-5.6 Sol

Replicate 3

CorrectUDT + standard tools
Answer2;-1
Workflow
Bedtools SlopbedExtract Genomic Dna 1Infer Tn5 fragment endpoint shifts
Total tokens
2.9M
Output
14.1K

Codex + DeepSeek-v4-pro-0813

3 replicates
Codex + DeepSeek-v4-pro-0813

Replicate 1

CorrectUDT + standard tools
Answer2;-1
Workflow
Add A Column1Converter Gz To UncompressedSelect columnsBedtools GetfastabedFasta Nucleotide Color Plot2 more tools
Total tokens
26.2M
Output
95.9K
Codex + DeepSeek-v4-pro-0813

Replicate 2

CorrectUDT + standard tools
Answer2;-1
Workflow
No Galaxy tool job recorded
Total tokens
4.6M
Output
38.6K
Codex + DeepSeek-v4-pro-0813

Replicate 3

CorrectUDT + standard tools
Answer2;-1
Workflow
Make ATAC endpoint pointsGet Flanks1Extract Genomic Dna 1Converter Interval To Bed 0Bedtools Getfastabed19 more tools
Total tokens
57.0M
Output
256.6K

Codex GPT-5.6 Luna

3 replicates
Codex GPT-5.6 Luna

Replicate 1

RerunCorrectUDT + standard tools
Answer2;-1
Workflow
Add a column1 → User-defined tool → datamash ops → Count1 → bedtools getfastabed → seq composition → fasta2tab
Total tokens
27.2M
Output
98.4K
Codex GPT-5.6 Luna

Replicate 2

CorrectUDT + standard tools
Answer2;-1
Workflow
Fragment basic diagnosticUDT rendering probeUDT rendering probe v2UDT rendering probe v3UDT rendering probe v421 more tools
Total tokens
24.4M
Output
77.7K
Codex GPT-5.6 Luna

Replicate 3

CorrectUDT + standard tools
Answer2;-1
Workflow
Fragment Structure DiagnosticExtract hg38 TSS BEDTSS Shift ProbeTSS Coverage Shift GridFragment Preview7 more tools
Total tokens
19.2M
Output
78.0K

Anycode

13/13 terminal item results

Codex GPT-5.5

3 replicates
Codex GPT-5.5

Replicate 1

Incorrect
Answer3;-3
Workflow
Local codePython
Codex GPT-5.5

Replicate 2

Correct
Answer2;-1
Workflow
Local codePython
Codex GPT-5.5

Replicate 3

Incorrect
Answer2;-2
Workflow
Local codeSamtoolsBedtoolsPython

Codex GPT-5.6 Sol

3 replicates
Codex GPT-5.6 Sol

Replicate 1

Correct
Answer2;-1
Workflow
Local codePython
Codex GPT-5.6 Sol

Replicate 2

Correct
Answer2;-1
Workflow
Local codePython
Codex GPT-5.6 Sol

Replicate 3

Correct
Answer2;-1
Workflow
Local codePython

Codex + DeepSeek-v4-pro-0813

3 replicates
Codex + DeepSeek-v4-pro-0813

Replicate 1

Incorrect
Answer6;-6
Workflow
Local codeSamtoolsBedtoolsPythonSciPy
Total tokens
2.7M
Output
40.3K
Codex + DeepSeek-v4-pro-0813

Replicate 2

Correct
Answer2;-1
Workflow
Local codeSamtoolsBedtoolsPythonpysam1 more tools
Total tokens
3.5M
Output
42.1K
Codex + DeepSeek-v4-pro-0813

Replicate 3

Correct
Answer2;-1
Workflow
Local codePythonpysam
Total tokens
3.3M
Output
38.8K

Codex GPT-5.6 Luna

3 replicates
Codex GPT-5.6 Luna

Replicate 1

Correct
Answer2;-1
Workflow
Local codeSamtoolsBedtoolsPythonpysam
Codex GPT-5.6 Luna

Replicate 2

Correct
Answer2;-1
Workflow
Local codePythonSamtoolspysamSciPy1 more tools
Codex GPT-5.6 Luna

Replicate 3

Incorrect
Answer2;-9
Workflow
Local codeSamtoolsBedtoolsPython

Codex GPT-6 Astra

1 replicate
Codex GPT-6 Astra

Replicate 1

Correct
Answer2;-1
Workflow
Local codePythonSamtools
Total tokens
108.5K
Output
1.6K