Question
I got a scATAC-seq fragment file (unknown.shift.frag.bed.gz) from a colleague who aligned it to the hg38 genome. However, they applied a non-standard shift to the reads instead of the typical +4/-5. Can you help figure out what it is? Report answer in the format "3;-3" (corresponding to values for 5' and 3' ends).
- Inferred answer
2;-1Uniquely inferred from submitted scores.
- Domain
- Epigenomics
- Question style
- Metadata Recovery
An answer can be uniquely inferred for 54 of 100 questions from submitted scores. Green: correct · Red: incorrect. For questions without a uniquely determined answer: Blue: matches prediction · Orange: differs from prediction. These are inferred answer checks, not official per-question grades.
Galaxy
12/12 terminal item results
UDT = user-defined tool: custom code executed by Galaxy. Route badges summarize analysis tools, excluding uploads and automatic conversions. How usage is counted.
Codex GPT-5.5
3 replicates
Codex GPT-5.5
Replicate 1
IncorrectUDT-only
Answer2;-2
WorkflowFragment summary diagnostic→Tn5 shift chr1 diagnostic→Tn5 shift multichrom final
- Total tokens
- 525.8K
- Output
- 11.0K
Codex GPT-5.5
Replicate 2
CorrectUDT-only
Answer2;-1
WorkflowATAC shift diagnostic→ATAC shift diagnostic v2→UDT smoke copy
- Total tokens
- 1.8M
- Output
- 19.1K
Codex GPT-5.5
Replicate 3
RerunIncorrectUDT-only
Answer6;-5
WorkflowATAC shift endpoint sequence diagnostic→ATAC shift candidate scorer→ATAC shift HINT bias validation→ATAC shift HINT native kmer validation
- Total tokens
- 1.7M
- Output
- 24.6K
Codex GPT-5.6 Sol
3 replicates
Codex GPT-5.6 Sol
Replicate 1
CorrectUDT + standard tools
Answer2;-1
WorkflowExtract Genomic Dna 1→Infer Tn5 shift from fragment-end sequence bias→Create centered fragment-end windows→Create left fragment-end windows→Locate Tn5 bias axis in centered endpoint windows→1 more tools
Codex GPT-5.6 Sol
Replicate 2
CorrectUDT + standard tools
Answer2;-1
WorkflowMake oriented endpoint windows→Extract Genomic Dna 1→Analyze endpoint sequence bias→Call nonstandard Tn5 shift
- Total tokens
- 914.3K
- Output
- 11.0K
Codex GPT-5.6 Sol
Replicate 3
CorrectUDT + standard tools
Answer2;-1
WorkflowBedtools Slopbed→Extract Genomic Dna 1→Infer Tn5 fragment endpoint shifts
- Total tokens
- 2.9M
- Output
- 14.1K
Codex + DeepSeek-v4-pro-0813
3 replicates
Codex + DeepSeek-v4-pro-0813
Replicate 1
CorrectUDT + standard tools
Answer2;-1
WorkflowAdd A Column1→Converter Gz To Uncompressed→Select columns→Bedtools Getfastabed→Fasta Nucleotide Color Plot→2 more tools
- Total tokens
- 26.2M
- Output
- 95.9K
Codex + DeepSeek-v4-pro-0813
Replicate 2
CorrectUDT + standard tools
Answer2;-1
WorkflowNo Galaxy tool job recorded
- Total tokens
- 4.6M
- Output
- 38.6K
Codex + DeepSeek-v4-pro-0813
Replicate 3
CorrectUDT + standard tools
Answer2;-1
WorkflowMake ATAC endpoint points→Get Flanks1→Extract Genomic Dna 1→Converter Interval To Bed 0→Bedtools Getfastabed→19 more tools
- Total tokens
- 57.0M
- Output
- 256.6K
Codex GPT-5.6 Luna
3 replicates
Codex GPT-5.6 Luna
Replicate 1
RerunCorrectUDT + standard tools
Answer2;-1
WorkflowAdd a column1 → User-defined tool → datamash ops → Count1 → bedtools getfastabed → seq composition → fasta2tab
- Total tokens
- 27.2M
- Output
- 98.4K
Codex GPT-5.6 Luna
Replicate 2
CorrectUDT + standard tools
Answer2;-1
WorkflowFragment basic diagnostic→UDT rendering probe→UDT rendering probe v2→UDT rendering probe v3→UDT rendering probe v4→21 more tools
- Total tokens
- 24.4M
- Output
- 77.7K
Codex GPT-5.6 Luna
Replicate 3
CorrectUDT + standard tools
Answer2;-1
WorkflowFragment Structure Diagnostic→Extract hg38 TSS BED→TSS Shift Probe→TSS Coverage Shift Grid→Fragment Preview→7 more tools
- Total tokens
- 19.2M
- Output
- 78.0K